Coding

Part:BBa_K3863004:Design

Designed by: LEONG WENG I   Group: iGEM21_PuiChing_Macau   (2021-09-26)


Phac-flag-PCT(optimized)-HA-his


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1708
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 87
    Illegal BamHI site found at 1506
    Illegal XhoI site found at 1030
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1060
    Illegal NgoMIV site found at 2125
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 251


Design Notes

By adding 6*his, it can be used to detect the protein via anti-polyhistidine-tag antibodies or alternatively by in-gel staining (SDS-PAGE).


Source

Phac (BBa_K2042001 from iGEM 2016 iGEM16_Evry) PCT (BBa_K1211001 from iGEM 2013 iGEM13_Yale)

References